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Image analysis, per-cycle basecalling and quality score assignment were made using Illumina Real Time Analysis software (Illumina, San Diego, CA). BCL files were converted to FASTQ format using Illumina's Off-Line Basecaller package.. 2009; 36:290-301. doi: 10.1016/j.molcel.2009.07.031. [PMC free article] [Google Scholar] 27. Meunier S.
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Genome sequencing was performed using Illumina technology (San Diego, CA, USA). The sequencing library preparation and sequencing of the whole genome was done by the Centre for Genomic Regulation (CRG, Barcelona, Spain) using an Illumina Hi-Seq Sequencing with 2 × 125 bp v4 chemistry.. 1998; 36:944-948. doi: 10.1128/JCM.36.4.944-948.1998.
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The final libraries were loaded into the cluster station (cBot, Illumina). and about 36% were finally assigned to a high-confidence PAS (as described in methods). Paired-end reads were 44 bp.